Genome wide identification of AGC kinase genes and their expression in response to heat and cold stresses in barley

AGC kinases are highly conserved regulators in a variety of cellular processes such as differentiation, proliferation, and growth. They known to play important roles stress hormonal responses, including ROS signaling. the main class protein plants, having central functions different stages plant In present study, analysis phylogenetic relationships, gene structures, chromosomal locations, synteny analysis, ontology, subcellular localization, expression kinase identified 28 genes barley. Phylogenetic tree grouped them into seven subfamilies, supported by exon-intron organization. Gene duplication indicated that tandom block events played an essential role expansion families The Real-time quantitative reverse transcription PCR (qRT-PCR) performed for HvAGC were largely expressed tissues roots, stems, leaves Azaran Jolgeh cultivars under heat cold stresses. results localization showed located on all chromosomes barley except chromosome 1. Genome evolution species was surveyed using identification orthologous paralogous genes. Identifying overlaps between clusters can enable us study function proteins species. To our knowledge, this is first detailed report bioinformatics Results revealed broad understanding family barley, which will be valuable improving varieties’ response Also, HvNDR6.2 utilized molecular markers three organs.